These results suggest that CsLHY-SRDX affects CsFT元 and CsAFT expression and causes photoperiod-insensitive floral transition without a severe defect in the circadian clock. Simultaneously with the flowering phenotype, CsFT元 and CsAFT were downregulated in CsLHY-SRDX transgenic plants. Thus, CsLHY-SRDX chrysanthemum showed photoperiod-insensitive floral transition, but further development of the capitulum was arrested, and anthesis was not observed. CsLHY-SRDX does not cause a severe defect in the circadian clock. These plants formed almost the same number of leaves before floral transition under SD and long-day conditions. chrysanthemum induced by constitutive expression of chimeric repressor CsLHY-SRDX. In adult plants, the phenotypes of 35Sp:c-Myc-CES-SRDX plants became even. Although the transcription of clock-related genes was conditionally affected, circadian rhythm was not completely disrupted in CsLHY-SRDX transgenic plants. Loss-of-function analysis and the use of a dominant repressor version revealed. We fused CsLHY to a gene encoding short transcriptional repressor domain ( SRDX) and constitutively expressed it in chrysanthemum. Here, we focused on a LHY/ CCA1-like gene called CsLHY in chrysanthemum. Meanwhile, the time-keeping mechanism that regulates the photoperiodic flowering in chrysanthemum is poorly understood. CsFT元 and CsAFT are induced under SD or a noninductive photoperiod, respectively, and their balance determines the floral transition and anthesis. In particular, MYC5-SRDX plants were male-sterile. transgenic lines expressing MYC5 fused to an SRDX (SUPERMAN repressive domain X) motif phenocopied mutants defective in JA signaling. We identified FLOWERING LOCUS T-LIKE 3 (FT元) and ANTI-FLORIGENIC FT/TFL1 FAMILY PROTEIN (AFT) as a florigen and antiflorigen, respectively, in a wild diploid chrysanthemum ( Chrysanthemum seticuspe f. Male sterility in Arabidopsis induced by overexpression of a MYC5-SRDX chimeric repressor Published in: Plant Journal, February 2015 DOI: 10.1111/tpj.12776. Chrysanthemum is a typical short-day (SD) plant and responds to shortening of day length by the transition from the vegetative to reproductive phase. In Arabidopsis, LATE ELONGATED HYPOCOTYL ( LHY) and CIRCADIAN CLOCK ASSOCIATED 1 ( CCA1) constitute the central oscillator, and their gain of function and loss of function disrupt the circadian clock and affect flowering time through FLOWERING LOCUS T ( FT), a gene encoding a florigen. A wide variety of physiological processes including flowering are controlled by the circadian clock in plants.
0 kommentar(er)
